During the last decade, many cartilage
tissue engineering strategies have been developed, being the stem cell-based
approach one of the most promising. Transforming Growth Factor-?3 (TGF-?3) and
Insulin-like Growth Factor-I (IGF-I) are key proteins on the regulation of
chondrogenic differentiation. Therefore, these two growth factors (GFs) were
immobilized at the surface of a single electrospun nanofibrous mesh (NFM)
aiming to differentiate human Bone Marrow-derived Mesenchymal Stem Cells
(hBM-MSCs). For that, the immobilization of defined antibodies (i.e.
anti-TGF-?3 and anti-IGF-I) allows the selective retrieval of the
abovementioned GFs from human platelet lysates (PL). Biochemical assays,
involving hBM-MSCs cultured on biofunctional nanofibrous substrates under basal
culture medium during 28 days, confirms the biological activity of bound TGF-?3
and IGF-I. Specifically, the typical spherical morphology of chondrocytes and
the immunolocalization of collagen type II confirmed the formation of a
cartilaginous ECM. Therefore, the proposed biofunctional nanofibrous substrate are
able to promote chondrogenesis.